Dark blue indicates well will be read. Running both standard spectrophotometer and microplate. Related Products: 96 Well Plate Reader. maximum-illumination-channels. Two high-energy xenon flash lamps: Reading capabilities. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . Highly selective for dsDNA over ssDNA or RNA. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. 100 fmol luciferase per well. Step 1: Capture antibody binds to ELISA plate wells. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. (Molecular Devices cat. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Pick the best speed, sensitivity, and price for your needs. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Products : General, FilterMax F5 reader. Software Validation. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. Our. Category 2 and 3 microplate readers use gradient heating as well. Wavelength(s):. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Description. 5 ng per well. to each well. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Dimensions: 18. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . 7°C (gradient heating design). Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 00 USD. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Features. Capabilities. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. For biochemical assays, the washer removes unbound material and unreacted reagents. 1. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. Temperature control range from Ambient +4º to 50ºC. Optional 16-way manifold. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. 5 fg per well to 0. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Enter the name of the plate type i. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. FlexIII. Materials. The CellTiter-Glo assay was used to measure ATP. 2% in. . The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. 1 mL/well of media. explore more. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. 5 µL of each sample was pipetted into a 96-well plate for PCR. Z. 2b,c. SpectraDrop High-Throughput Screening (HTS) Kit. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. White 96-well platePlate formats. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Selects either 96-well or 384-well plate for a stand-alone read. Cell viability and cytoxicity assays. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 057, depending on lot number. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. 5 ng per well. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Users can choose to process all plates within the magazine or select a specific number. adjust the reader. Selects either 96-well or 384-well plate for a stand-alone read. Includes PC and software. Compare. Microplate Readers. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. 305 reference level saturation. The plate reader optics are tunable for. 96- and 384-well microplates. 1 – 2. 000 to 2. We would like to show you a description here but the site won’t allow us. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . Multimodal plate reader: Yes: 25 mm: Yes—Max. Molecular Devices (UK) Ltd. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. minutes and begin reading microplates with seven mouse clicks. µl) was added to each well and incubated at 37°C for 1 h. Browse for different fluorescent readers below and compare across different instrument suppliers. Important things to consider when purchasing a multimode microplate reader. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. $14,995. #R8195. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Features. Plate Shaking. 50/plate. Validation made easy -1) Select the correct plate and the wells that you want to measure. Click on the crosshair and move it so that it is centered over the well image. 40266. Mode Plate Reader. . Temperature control: 4°C above ambient to 45°C. 00 . The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. We would like to show you a description here but the site won’t allow us. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Compare this item. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. Molecular Devices' microplate readers. C. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. VMax . No tools required to assemble. It also integrates with the powerful SoftMax Pro Software for. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Microplate readers, SpectraMax®, with SpectraDrop. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. The StakMax stacker also allows users to feed plates into the loading area manually. Click Create. Inconsistent results: cannot determine whole well confluence – well after well. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. x. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Optimized for fluorescence microplate readers. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. What are the dimensions of the plate? Code. An entire 96-well plate can be processed in less than six seconds. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Order Now. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. 1 OD at 230 nm. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. 2 with 1000 cells. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Figure 1-2 Match chamfered corners of slide and adapter 2. The drawing shows a standard microplate of 15 mm height covered with a lid. 5L) MultiWash+. Gemini EM Reader scans. 000 to 2. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. 300 µL of Detergent Compatible Bradford reagent was added to each well. Microplate Capacity. 25% and 0. • Photometric range: 0. This reader also makes use of a unique temperature-independent methodology for path length correction. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. High-throughput, automated dynamic light scattering. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 000 – 4. 1. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Page 1 of 8 . Two different agonists, acetylcholine and carbachol, were tested. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. 96-well-plate-scan-time-2-colors-1-site-well. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. This reader's dual monochromators for. U-bottom: 30-40 µl. The system features 96, 384,. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. The Versamax Microplate Reader is built around a grating monochromator. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. x. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. Select models. one 96-well sample plate can be controlled from 15–40°C,. Can read 96 well plates (optional 384 well). Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. Other technologies such as imaging. 1. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. This page contains the. With various on-board features and capabilities, the ThermoMax. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. The VICTOR Nivo. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. UV Plate 96 Well, Flat Bottom, Cs 50. In addition, the iD5 reader measures TRF and FP and. Mix samples in microplate wells by user-programmable Automix feature. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Shaking: Linear, orbital, and double orbital. Microplates, cuvettes (via adapter) Detectors. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 90349). The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. B. 9. Lucid Scientific. x. 9. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Consequently, FLIPR has been used. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Place the microplate in the drawer. ©2022 Molecular Devices - All rights. Figure 2-23: Bottle holder To install the bottle holder: 1. 96 well plate injection time with SpectraMax® L plate reader. Visit the supplier page or submit a quote to learn more. S. g. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Used. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Packed 25 plates per sleeve, 2 sleeves per case. Absorbance Linearity: 2. SpectraMax i3x Multi-Mode Microplate Reader. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. 76358-624. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Silica excitation fiber, glass emission fiber. 005 OD Normal and Rapid modes, 96- and 384-well plates;. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Description. Luminescence applications and assays. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. x. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Place the microplate in the drawer. Microplates, cuvettes (via adapter) Detectors. Absorbance equation. EMax. 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. Select up to 3 products. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. absorbance microplate reader GEA. M2. Unparalleled performance on a personalized platform. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Or scan up to 384 wells at any time to determine the best assay conditions. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . We would like to show you a description here but the site won’t allow us. 190. 306 plate air cal. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. $488. They can be read by most microplate readers. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. 190. x. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Your applications, your modes, your choice. Detection of KASP genotyping reactions. Plate. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Top and bottom reads are. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. Contact our support team with your questions regarding Molecular Devices products and applications. 45 µm PVDF. 500 USD. Three low volume 24-well bottom slides. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. No. 2. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. and the A1 corner of the plate adapter (Figure 1-2). The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. as well as all other Molecular Devices' microplate readers. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. 96-, 384-, and 1536-well microplates. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). Growth medium was then replaced with fatty acid uptake solution. Controlled by the computer. Values are averages from 5 clean plates, each filled with 200 µL water/well. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. No additional manuals are available. to each well. Primary considerations when choosing a microplate reader are throughput and flexibility. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. 1% in white 96- and < 0. Two different agonists, acetylcholine and carbachol, were tested. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. 00. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. 20 µL (96-well plates) or 10 µL (384. PURPOSE. The picked. measurements in compatible Molecular Devices® microplate readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. x. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Find our technical support information along with contact details. Sample by eye within . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Bowman Dispensers. x. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Answer. Intuitive graphical user interface makes each assay extremely easy to set. Instrument settings can. Access this by going to. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Fluorescence Microplate Reader Comparison Chart. x. 500 OD ± 3. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 0, or. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. 0025 OD from 0. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. An industry-renowned instrument for monitoring GPCRs and ion channels. View Product. The color touch screen provides a visual user interface, making programming fast and intuitive. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. It has a 340-750nm Spectral Range. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. #CA961) Methods. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. SoftMax Pro GxP Software version 5. 2. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Other. #C1500) Control standard endotoxin (ACC cat.